Preparation of polyhydroxyalkanoates using <i>Pseudomonas helmanticensis</i> in non-sterile media containing glycerol and sodium dodecyl sulfate

Biosynthetically-produced Pseudomonas poly-3-hydroxyalkanoates (PHAs) are a promising substitute for conventional plastics. Costs involved with the production of PHAs can be reduced by optimizing power consumption, which achieved using nutrient media without preliminary steam sterilization. Cultivation bacteria resistant to sodium dodecyl sulfate (SDS) on SDS-containing non-sterile yields biomass consisting predominantly PHA producer. SDS plays role an antimicrobial agent that inhibits growth foreign microorganisms. In this work, SDS-resistant culture helmanticensis and containing glycerol were used. The concentrations carbon (glycerol) nitrogen sources optimized experiment performed according central composite rotatable design. concentration substrate C C/N ratio between content varied. dependence degree conversion in was derived R programming environment. constructed model adequately describes experimental data at significance level 0.05 (adequacy variance regression equation 4.1×10 -2 ; 2 =0.98). According model, equals 6.9±0.4%. Under conditions (0.61 g/L source; 8.4 glycerol; 96 h), P. converts 7.0% average monomer unit length. Using 16S rRNA metagenomic assay, proportion cultures 0.5 shown 2%.